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Table 2 Neuroinvasiveness testing of the mutants by i.p. inoculation of 3-week-old mice

From: The mutation of Japanese encephalitis virus envelope protein residue 389 attenuates viral neuroinvasiveness

Virus dilution multiple

Neuroinvasiveness assay in 3-week-old mice (death/total)

WT

D389S

D389G

D389H

100

NA

6/6

5/6

2/6

101

6/6

6/6

2/6

1/6

102

5/6

1/6

0/6

0/6

103

1/6

0/6

0/6

0/6

104

2/6

0/6

0/6

0/6

105

0/6

NA

NA

NA

Initial titer of virus (PFU/ml)

1.18 × 106

1.3 × 107

8.9 × 106

9 × 106

LD50(PFU)

1.18 × 103

1.63 × 105

1.03 × 106

>4.5 × 106

  1. NA: not available, represent the experiments were not carried out
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Virology Journal

ISSN: 1743-422X