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Table 1 Percentage of the genome covered (> 30X) in the three independent replicates, concatenating two replicates (2X) and concatenating three replicates (3X) in the specimens that overcame the quality threshold when concatenating. The replicates which were randomly selected as references are shaded

From: A solution to achieve sequencing from SARS-CoV-2 specimens with low viral loads: concatenation of reads from independent reactions

Sample ID

Ct

1X

2X

3X

A

B

C

R-3

38.3

87.63

72.65

94.28

95.2 (AB)

99.02

R-5

33

90.28

68.06

87.11

97.47 (AC)

98.35

R-7

33.3

67.06

51.97

69.81

84.56 (BC)

92.22

R-9

35

83.62

89.34

89.15

97.59 (BC)

98.51

R-20

34

80.41

85.46

83.84

92.17 (BC)

94.27

R-28

34

96.52

95.79

75.63

98.69 (BC)

99.59

R-29

33

78.67

76.65

72.73

94.04 (AC)

96.4