Fig. 3

Neutralizing ability of monoclonal antibody 72A1 against CNE2-EBV-GFP infection of B cells determined by FCM and HCIS. A Neutralizing ability of 72A1 determined by FCM using doses of CNE2-EBV-GFP with different levels of infection efficiency (10%, 20% and 40%). Data are shown as mean ± SEM (n = 2). Curves were fit using four parameters nonlinear regression. B Neutralizing ability of 72A1 determined by HCIS using doses of CNE2-EBV-GFP with different levels of infection efficiency (10%, 20% and 40%). Data was shown as mean ± SEM (n = 2). Curves were fit using four parameters nonlinear regression. C Half maximal neutralizing concentration (NC₅₀) of 72A1 determined by FCM and HCIS, for each dose of virus. D Correlation of NC₅₀ values between FCM and HCIS assays