Characterization and determination of holin protein of Streptococcus suis bacteriophage SMP in heterologous host

Table 1 Relative quantitation of HolSMP using the comparative C_T method

Time (min)	HolSMP Average C_T	GAPDH Average C_T	ΔC_T HolSMP- GAPDH^a	ΔΔC_T ΔC_T-ΔC_T, _{0 min}^b	HolSMP Rel. to 0 min^c
0	30.61 ± 0.09	16.64 ± 0.03	13.97 ± 0.09	0.00 ± 0.09	1.0 (0.9-1.1)
5	30.66 ± 0.03	16.83 ± 0.03	13.83 ± 0.04	-0.14 ± 0.04	1.1 (1.07-1.13)
10	29.60 ± 0.05	18.40 ± 0.03	11.20 ± 0.06	-2.77 ± 0.06	6.8 (6.5-7.1)
15	24.54 ± 0.03	16.30 ± 0.05	8.24 ± 0.06	-5.73 ± 0.06	53.1 (50.9-55.3)
20	21.27 ± 0.02	16.38 ± 0.04	4.89 ± 0.04	-9.08 ± 0.04	541.2 (526.4-556.4)
40	17.69 ± 0.03	18.49 ± 0.03	-0.80 ± 0.04	-14.77 ± 0.04	27939.1 (27175.1-28724.6)
60	16.10 ± 0.02	18.61 ± 0.05	-2.51 ± 0.05	-16.48 ± 0.05	91405.9 (88292.3-94629.3)
80	16.42 ± 0.03	18.55 ± 0.02	-2.12 ± 0.04	-16.09 ± 0.04	69754.6 (67847.1-71715.6)
100	15.26 ± 0.07	16.56 ± 0.04	-1.30 ± 0.08	-15.27 ± 0.08	39511.9 (37380.6-41764.8)
120	17.94 ± 0.03	18.68 ± 0.01	-0.74 ± 0.03	-14.71 ± 0.03	26801.0 (26249.5-27364.2)

a. The ΔC_T value is determined by subtracting the average GAPDH C_T value from the average HolSMP C_T value. The standard deviation of the difference is calculated from the standard deviation of the HolSMP and GAPDH values.
b. The calculation of ΔΔC_T involves subtraction by the ΔC_T calibrator value. This is subtraction of an arbitrary constant, so the standard deviation of ΔΔC_T is the same as the standard deviation of the ΔC_T value.
c. The range given for HolSMP relative to 0 min is determined by evaluation the expression: 2^-ΔΔCT with ΔΔC_T + s and ΔΔC_T - s, where s = the standard deviation of the ΔΔC_T value.

ISSN: 1743-422X