Figure 6

HolSMP functions as a holin to complement S-negative phage λ c I857 Sam 7. (A) Host cell lysis by phage λ c I857 Sam 7 requires a permissive host, featuring supF, such as VCS257. (B) Plaques will not be produced on plates with nonermissive host BL2(DE3)pLysS. (C) With the expression of sub-lethal levels of HolSMP, large and clear plaques were observed on plate with host BL2(DE3)pLysS (pEXH1).