Figure 5

Co-expression of HolSMP and LySMP results rapid lysis of BL21(DE3) host cells. The growth of BL21(DE3) cells were monitored by absorbance determination after cotransformation with pEX + pACEXL (expressing only LySMP), pEXH1 + pACEX (expressing HolSMP), and pEXH1 + pACEXL (expressing both HolSMP and LySMP), respectively.