Figure 2

Detection of transcription and expression of gene HolSMP in S. suis after phage infection. (A) Reverse transcription PCR was performed to evaluate HolSMP transcript levels. The quantities of HolSMP transcripts in each sample were compared, while the house-keeping gene, GAPDH, was employed as a reference for normalization of samples. (B) Western blotting was performed on the membrane fraction extracted from phage infected host cells.