Figure 2

Immunofluorescence staining showing stable intracytoplasmic expression of NS3 helicase specific antibody and control antibody in Huh-7.5 cells. Stable Huh-7.5 cells were seeded in 2-well chamber slides and after 48 hours cells were stained with phycoerythrin conjugated anti-human IgG1 antibody at a dilution of 1:50. After this step, cells were counterstained with hoechst dye. A: Negative staining of control Huh 7.5 cells. B: Huh 7.5 cells stably expressing a control antibody. C: Huh 7.5 cells stably expressing NS3 antibody.