Prevention of genital herpes in a guinea pig model using a glycoprotein D-specific single chain antibody as a microbicide

Table 1 Degenerate PCR primers used for amplification of V_L (kappa) and V_H (gamma).

Nomenclature	Primer sequences used for PCR reactions
Signal sequence/framework primers
Kappa 1	GGTGATATCGTGATRACMCARGATGAACTCTC
Kappa 2	GGTGATATCWTGMTGACCCAAWCTCCACTCTC
Kappa 3	GGTGATATCGTKCTCACYCARTCTCCAGCAAT
Kappa 4	CTGWTGTTCTGGATTCCTG
Kappa 5	GTGCTCTGGATTCGGGAA
Kappa 6	TCAGCTTCYTGCTAATCAGTG
Kappa 7	TGGGTATCTGGTRCSTGTG
Kappa 8	GTTTCMAGGTRCCAGATGT
Kappa 9	TGTTTTCAAGGTRCCAGATGT
Kappa 10	CTSTGGTTGTCTGGTGTTGA
Kappa 11	TGCTKCKCTGGGTTCCAG
C region kappa primer	TGGTGGGAAGATGGA
Signal sequence/framework primers
Gamma 1	GAGGTGAAGCTGCAGGAGTCAGGACCTAGCCTGGTG
Gamma 2	AGGTVMAACTGCAGVAGTCWGG
Gamma 3	AGGTVVAGCTGCAGVAGTCWGG
Gamma 4	ACTGCAGGTRTCCACTCC
Gamma 5	RCTACAGGTGTCCACTCC
Gamma 6	GCYACAGMTGTCCACTCC
Gamma 7	ACTGCAGGTGTCCTCTCT
Gamma 8	RCTRCAGGYGTCCACTCT
Gamma 9	CCAAGCTGTGTCCTRTCC
Gamma 10	TGTTGACAGYCVTT CCKGGT
Gamma 11	TAYTTTAAAARGTGTCMAGTGT
Gamma 12	CTYTTAAAAGGKGTCCAGWG
Gamma 13	CYTTTAMATGGTATCCAGTGT
Gamma 14	ATGGCAGCWGCYCAAAG
Gamma 15	CTTTTAAAAGWTGTCCAGKGT
Gamma 16	CTTCCTGATGGCAGTGGTT
C region gamma primer	TAACCCTTGACCAGGCATCC

Key to degenerate nucleotides: R = A+G; M = A+C; W = A+T; K = G+T; S = G+C; Y = C+T; H = A+T+C; B = G+T+C; D = G+A+T; N = A+C+G+T; V = G+A+C

ISSN: 1743-422X